On October 2nd, local time, the second round of the 2025/26 Europa League kicked off. After 90 minutes of intense competition, Roma suffered their first defeat at home, losing 0-1 to Lille.

At the beginning of the game, Roma missed the opportunity due to defensive errors: in the 6th minute, Konstantinos Tsimikas should have received Mario Hermoso's pass but accidentally missed the ball, allowing Haakon Haraldsson to pass the ball to Thomas Meunier and Felix Correa in the penalty area and then volley the ball into the goal.

From then on, both teams exchanged offense and defense. In the 25th minute, Bryan Cristante crossed from the top of the key, and Lorenzo Pellegrini's long-range shot was weakly caught by Berk Özer. In the 30th minute, Lille's frontcourt continued to cooperate, and Oussama Al-Sahraoui's shot from the left side of the penalty area was slightly wide of the far corner. In the 35th minute, Wesley sent a cross from the right, and Tsimikas' header in the penalty area was blocked by Meunier. Matias Sule and Tsimikas' subsequent shots were blocked, and Nir El Ainawi's close-range shot was successfully saved by Özer.

After the break, Lille once again took the lead: in the 54th minute, Nabil Bentalib's long pass from midfield found Giroud, who faced Miller Svilar with a one-on-one shot, but his shot was saved. In the 56th minute, Sule took a corner kick from the right side, and Evan Ferguson's header hit the crossbar. In the 74th minute, Ainawi passed the ball to the right in the frontcourt, and Sule's long-range shot was confiscated by Özer.

In the 79th minute, Sule's cross from the right corner resulted in a handball foul by Aissa Mendy. Dovbeek's subsequent penalty kick was saved brilliantly by Özer. Although Lille committed two subsequent fouls, giving Dovbeek and Sule opportunities to take penalties, Özer's effort kept the goal intact. At the end of the match, Roma lost to Lille 0-1.